Gas chromatography of fatty acid amides

Analysis of fatty acid composition of Withania coagulans fruits by gas chromatography/mass spectrometry

Background and objectives: Withania coagulans Dunal (Solanaceae) fruits are recommended to treat asthma, dyspepsia, biliousness, flatulent colic, liver complaints, intestinal infections, strangury, wounds and as diuretic, emetic and sedative agent in Indian traditional system of medicine. The objective of this study was to describe the systematic fatty acid composition...

Preparation of fatty acid methyl esters for gas-liquid chromatography.

A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol....

Microsomal synthesis of fatty acid amides.

A microsomal system is described which catalyzes the formation of fatty acid amides of ethanolamine and of several pharmacologically active amines. Although the system has no dependance on external energy sources and has a high K, for the amine substrate, it has several characteristics which differ markedly from an amide hydrolytic system in the microsomes. A possible mechanism for the formatio...

Cytotoxic fatty acid amides from Xenorhabdus.

Fatty Acid Amides of Ethanolamine in Mammalian Tissues.

While investigating the metabolism of ethanolamine by rat liver microsomes, we observed an incorporation of this amine into microsomal lipid that was dependent on the presence of a fatty acid as cosubstrate (1). It was shown that the product of the enzymatic reaction was the fatty acid amide of ethanolamine and that the reaction was synthetic, since little hydrolysis of added fatty acid amide c...